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Active Integrin Alpha M (CD11b)

ITGaM; ITG-AM; CR3A; MAC-1; MAC1A; MO1A; Complement Component 3 Receptor 3 Subunit; Cell surface glycoprotein MAC-1 subunit alpha; Neutrophil adherence receptor

ACTIVITY TEST

Integrin Alpha M (ITGaM, also known as CD11b) is a transmembrane subunit of the heterodimeric integrin receptor Mac-1 (CD11b/CD18), widely expressed on myeloid cells including macrophages, neutrophils, and dendritic cells. It mediates cell adhesion to extracellular matrix proteins (e.g., fibrinogen, ICAM-1) and regulates phagocytosis, inflammation, and immune cell recruitment, playing critical roles in host defense against pathogens and tissue repair. ITGaM binds to complement component C3 (especially its activated fragment C3b), facilitating opsonophagocytosis and amplifying innate immune responses.Briefly, C3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ITGaM-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately.Measured by its binding ability in a functional ELISA. When Recombinant ITGaM is lmmobilized at 2 µg/mL(100 uLwell), the concentration of C3 that produces 50% optimal bindingresponse is found to be approximately 0.130 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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