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Active Bone Morphogenetic Protein 9 (BMP9)

GDF2; Growth Differentiation Factor 2

ACTIVITY TEST

Bone Morphogenetic Protein 9 (BMP9), a member of the Transforming Growth Factor Beta(TGF-b) superfamily, is predominantly expressed in the liver and endothelial cells. It exerts pivotal roles in regulating angiogenesis, glucose metabolism, and bone formation. As a multifunctional cytokine, BMP9 transmits signals via type I and type II serine/threonine kinase receptors on the cell surface, thereby modulating a variety of physiological and pathological processes. Notably, it binds to Activin A Receptor Type II A (ACVR2A) with high affinity, which initiates downstream SMAD-dependent signaling pathways.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse BMP9 and recombinant human ACVR2A . Briefly, biotin-linked BMP9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to ACVR2A-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant mouse BMP9 and recombinant human ACVR2A was shown in Figure 1, the EC50 for this effect is 0.17 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Metabolism Bone morphogenic protein 9 is a novel thermogenic hepatokine secreted in response to cold exposure Pubmed:35063533
Heliyon Scaffolds of Bioactive Glass (Bioglass®) Combined with Recombinant Human Bone Morphogenetic Protein-9 (rhBMP-9) for Tooth Extraction Site Preservation Pubmed:35600439
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB728Mu02 Recombinant Bone Morphogenetic Protein 9 (BMP9) Positive Control; Immunogen; SDS-PAGE; WB.
RPB728Mu03 Recombinant Bone Morphogenetic Protein 9 (BMP9) Positive Control; Immunogen; SDS-PAGE; WB.
APB728Mu03 Active Bone Morphogenetic Protein 9 (BMP9) Cell culture; Activity Assays.
RPB728Mu01 Recombinant Bone Morphogenetic Protein 9 (BMP9) Positive Control; Immunogen; SDS-PAGE; WB.
PAB728Mu02 Polyclonal Antibody to Bone Morphogenetic Protein 9 (BMP9) WB; IHC; ICC; IP.
PAB728Mu01 Polyclonal Antibody to Bone Morphogenetic Protein 9 (BMP9) WB; IHC; ICC; IP.
SEB728Mu ELISA Kit for Bone Morphogenetic Protein 9 (BMP9) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB728Mu CLIA Kit for Bone Morphogenetic Protein 9 (BMP9) Chemiluminescent immunoassay for Antigen Detection.
LMB728Mu Multiplex Assay Kit for Bone Morphogenetic Protein 9 (BMP9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.