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Active Interleukin 6 Receptor (IL6R)

CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80

ACTIVITY TEST

IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J Orthop Res Interleukin‐6 and interleukin‐6 receptor expression, localization, and involvement in pain‐sensing neuron activation in a mouse intervertebral disc injury model PubMed: 26010074
Gene.  The IL6R gene polymorphisms are associated with sIL-6R, IgE and lung function in Chinese patients with asthma Pubmed:26997259
Cytokine Interleukin-6 participates in human pancreatic stellate cell activation and collagen I production via TGF-¦Â1/Smad pathway 33893003
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB815Mu02 Recombinant Interleukin 6 Receptor (IL6R) Positive Control; Immunogen; SDS-PAGE; WB.
APB815Mu02 Active Interleukin 6 Receptor (IL6R) Cell culture; Activity Assays.
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