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Active Versican (VCAN)

CSPG2; VS; ERVR; PG-M; WGN1; Chondroitin Sulfate Proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate-binding; Large fibroblast proteoglycan

ACTIVITY TEST

Versican (VCAN) is a large extracellular matrix proteoglycan that plays critical roles in tissue development, inflammation, and cell adhesion by interacting with hyaluronan and other ECM components. It regulates tissue hydration, structural integrity, and immune cell migration, contributing to cardiovascular disease, cancer progression, and fibrosis through modulation of cell proliferation and matrix remodeling. Proteolytic cleavage of VCAN releases bioactive fragments that influence cellular signaling. Importantly, VCAN binds and sequesters TGFβ1 in the ECM, controlling its bioavailability; upon VCAN cleavage, TGFβ1 is released, activating downstream signaling pathways involved in fibrosis, immune regulation, and tissue repair.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse VCAN and recombinant dog TGFb1. Briefly, VCAN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TGFb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VCAN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse VCAN and recombinant dog TGFb1 was shown in Figure 1, the EC50 for this effect is 0.005ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Plos one Tumor-Produced Versican V1 Enhances hCAP18/LL-37 Expression in Macrophages through Activation of TLR2 and Vitamin D3 Signaling to Promote Ovarian Cancer Progression In Vitro PubMed: PMC3570526
Clinica Chimica Acta Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955
Journal of Cancer Research and Clinical Oncology Clinical significance of circulatory microRNA-203 in serum as novel potential diagnostic marker for multiple myeloma
RNA Biology Targeting of stromal versican by miR-144/199 inhibits multiple myeloma by downregulating FAK/STAT3 signaling Pubmed: 31532704
miR-124-3p and miR-181a-5p Mediate AT1-Receptor Autoantibody Induced Fetal Rat Cardiac Remodeling via Increased VCAN Expression
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB817Mu02 Recombinant Versican (VCAN) Positive Control; Immunogen; SDS-PAGE; WB.
APB817Mu01 Active Versican (VCAN) Cell culture; Activity Assays.
RPB817Mu01 Recombinant Versican (VCAN) Positive Control; Immunogen; SDS-PAGE; WB.
PAB817Mu01 Polyclonal Antibody to Versican (VCAN) WB
PAB817Mu02 Polyclonal Antibody to Versican (VCAN) WB; IHC; ICC; IP.
SEB817Mu ELISA Kit for Versican (VCAN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB817Mu Multiplex Assay Kit for Versican (VCAN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.