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Active Fetuin B (FETUB)

FETU-B; 16G2; Gugu; IRL685; Fetuin-like protein IRL685

ACTIVITY TEST

Fetuin B (FETUB) is a secreted cystatin-family glycoprotein regulating extracellular matrix turnover. It functionally interacts with Collagen Type I Alpha 2 (COL1A2). Their crosstalk tunes type I collagen synthesis and fibril assembly, participating in fibrotic progression and stromal microenvironment remodeling.To detect the activity of recombinant FETUB, a functional ELISA assay was performed to evaluate the interaction between recombinant bovine FETUB and recombinant COL1A2. Briefly, COL1A2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FETUB-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL1A2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. When recombinant FETUB is lmmobilized at 2 µg/mL(100 uLwell), the concentration of COL1A2 that produces 50% optimal binding response is found to be approximately 1.308 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Endocrine Serum fetuin B level increased in subjects of nonalcoholic fatty liver disease: a case-control study pubmed:27628583
Endocrine Increased serum levels of fetuin B in patients with coronary artery disease pubmed:28822077
DIABETES & METABOLISM Plasma fetuin-B concentrations are associated with insulin resistance and first-phase glucose-stimulated insulin secretion in individuals with different degrees of … Pubmed:29514765
Clinical & Investigative Medicine Serum level of fetuin B is associated with osteoporosis: a 4-year prospective study in China Pubmed:29603688
American Journal of Translational Research Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice Pubmed: 30972177
Endocrine Connections Increased serum fetuin-B concentration is associated with HOMA-β and indices of liver steatosis in women with polycystic ovary syndrome: a pilot study Pubmed: 31307012
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555
J Nephrol Citrate high volume on-line hemodiafiltration modulates serum Interleukin-6 and Klotho levels: the multicenter randomized controlled study ¡°Hephaestus¡± 33559851
Nutrients High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese … 34684637
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
APB860Bo01 Active Fetuin B (FETUB) Cell culture; Activity Assays.
RPB860Bo01 Recombinant Fetuin B (FETUB) Positive Control; Immunogen; SDS-PAGE; WB.
RPB860Bo02 Recombinant Fetuin B (FETUB) Positive Control; Immunogen; SDS-PAGE; WB.
PAB860Bo01 Polyclonal Antibody to Fetuin B (FETUB) WB; IHC; ICC; IP.
MAB860Bo21 Monoclonal Antibody to Fetuin B (FETUB) WB; IHC; ICC; IP.
SEB860Bo ELISA Kit for Fetuin B (FETUB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB860Bo Multiplex Assay Kit for Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMB860Bo Multiplex Assay Kit for Fetuin B (FETUB) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.