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Active Interferon Tau (IFNt)

IFN-T1; TP1; OTP; Trophoblastin; Interferon Tau-1; Trophoblast Protein 1; Trophoblast Antiluteolytic Protein; Antiluteolysin

ACTIVITY TEST

Interferon tau (IFNτ), a ruminantspecific typeI interferon secreted by conceptus trophectoderm, serves as the core signal for maternal pregnancy recognition. It triggers JAKSTAT cascades to exert immunomodulatory, antiproliferative and antiviral bioactivities. Beyond canonical receptor engagement, IFNτ exhibits direct physicalbinding activity toward 3Mercaptopyruvate Sulfurtransferase (MST).
Thus a functional ELISA assay was conducted to detect the interaction of recombinant IFN-τ and recombinant MST. Briefly, IFN-τ was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μL were then transferred to MST-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IFN-τ pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant IFN-τ and recombinant MST was shown in Figure 1, the EC50 for this effect is 0.426 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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