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Active Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

ACTIVITY TEST

Interleukin‑28B (IL28B), also designated IFN‑λ3, belongs to class‑II cytokine superfamily and exerts antiviral, antitumor and immunomodulatory bioactivities. It primarily binds IFNLR1 with high affinity and subsequently recruits IL10RB to assemble a functional heterodimeric receptor complex via protein‑protein interaction. This binding event triggers downstream JAK‑STAT signaling cascades, inducing interferon‑stimulated genes and mediating mucosal immune defense against invading pathogens.
Thus a functional ELISA assay was conducted to detect the interaction of recombinant IL28B and recombinant IFNLR1. Briefly, IL28B was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μL were then transferred to IFNLR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant IL28B and recombinant IFNLR1 was shown in Figure 1, the EC50 for this effect is 0.132µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Antimicrobial Chemotherapy Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV PubMed: 22294646
Cytokine Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. Pubmed: 24041672
Hepatology Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus Pubmed:25130193
Cytokine. Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis Pubmed:25461389
J Biomed Res.  Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … pmc:PMC4726832
Journal of Biomedical Research Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients PMC4726832
LUPUS Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus Pubmed: 30336754
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EPC028Bo61 Eukaryotic Interleukin 28B (IL28B) Positive Control; Immunogen; SDS-PAGE; WB.
APC028Bo61 Active Interleukin 28B (IL28B) Cell culture; Activity Assays.