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Active Neuregulin 4 (NRG4)

HRG4; Pro-NRG4; Pro-neuregulin-4, membrane-bound isoform

ACTIVITY TEST

Neuregulin 4 (NRG4) is a member of the neuregulin family of growth and differentiation factors, structurally related to epidermal growth factor (EGF). It is primarily expressed in brown adipose tissue and plays critical roles in regulating energy metabolism, thermogenesis, and adipocyte function. NRG4 acts as a ligand for ErbB receptor tyrosine kinases, especially ErbB4, activating downstream signaling pathways involved in cell survival, proliferation, and metabolic homeostasis. Emerging evidence links NRG4 dysfunction to obesity, diabetes, and metabolic disorders, highlighting its potential as a biomarker and therapeutic target for metabolic diseases. Unlike NRG1, NRG4 does not bind directly; both activate ErbB receptors but via distinct binding preferences and tissue distributions.To detect the activity of recombinant NRG4 , a functional ELISA assay was performed to evaluate the interaction between recombinant human NRG4 and recombinant human NRG1. Briefly, NRG4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NRG1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NRG4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse NRG4 and recombinant human NRG1 was shown in Figure 1, the EC50 for this effect is 0.149 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Metabolism A case–control study: Association between serum neuregulin 4 level and non-alcoholic fatty liver disease PubMed: 26476959
Diabetes Research and Clinical Practice Comparison of serum NeureCavia (Guinea pig )lin 4 (Nrg4) levels in adults with newly diagnosed type 2 diabetes mellitus and controls without diabetes Pubmed:27329015
Scientific Reports Short-chain fatty acids and inulin, but not guar gum, prevent diet-induced obesity and insulin resistance through differential mechanisms in mice. pubmed:28733671
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APC174Mu01 Active Neuregulin 4 (NRG4) Cell culture; Activity Assays.
RPC174Mu01 Recombinant Neuregulin 4 (NRG4) Positive Control; Immunogen; SDS-PAGE; WB.
RPC174Mu02 Recombinant Neuregulin 4 (NRG4) Positive Control; Immunogen; SDS-PAGE; WB.
PAC174Mu01 Polyclonal Antibody to Neuregulin 4 (NRG4) WB; IHC; ICC; IP.
PAC174Mu02 Polyclonal Antibody to Neuregulin 4 (NRG4) WB; IHC; ICC; IP.
SEC174Mu ELISA Kit for Neuregulin 4 (NRG4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC174Mu Multiplex Assay Kit for Neuregulin 4 (NRG4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.