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Active Allograft inflammatory factor 1 (AIF1)
IBA1; IRT1; Daintain; Protein G1; Ionized calcium-binding adapter molecule 1; G1
- Product No.APC288Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 348
US$ 870
US$ 1740
US$ 5220
US$ 13050
For more details, please contact local distributors!
ACTIVITY TEST
Lonized Calcium-binding Adapter Molecule 1 (IBA1), a 17-kDa EF-hand calcium-binding protein, serves as a gold-standard marker for microglia in the central nervous system (CNS). Predominantly expressed in microglia and marginally in peripheral macrophages, it mediates calcium-dependent cytoskeletal reorganization, governing microglial migration, phagocytosis of damaged neurons, and pro-inflammatory cytokine secretion. This protein is pivotal for CNS immune surveillance and homeostasis; its elevated expression is tightly linked to microglial activation in neuroinflammatory conditions and neurodegenerative diseases such as multiple sclerosis and amyotrophic lateral sclerosis. Additionally, IBA1 coordinates with actin-regulatory proteins to modulate microglial morphological transitions during immune responses. IBA1 interacts with ARPC4 to facilitate actin polymerization and modulate microglial migratory capacity in neuroinflammation.Thus a functional ELISA assay was conducted to detect the interaction of recombinant IBA1 and recombinant ARPC4. Briefly, IBA1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ARPC4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IBA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant IBA1 and recombinant ARPC4 was shown in Figure 1, the EC50 for this effect is 17.38µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Biosci Trends. | Allograft inflammatory factor-1 in the pathogenesis of bleomycin-induced acute lung injury Pubmed:26911661 |
| Cardiovasc Pathol | A novel role for the Krüppel-like factor 14 on macrophage inflammatory response and atherosclerosis development pubmed:27923151 |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease |
| 博士论文 | INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA |