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Active Apolipoprotein M (APOM)

Apo-M; G3a; NG20; Protein G3a

ACTIVITY TEST

Apolipoprotein M (APOM) is an approximately 25 kDa variably glycosylated protein that adopts a beta-barrel structure characteristic of lipocalin family proteins. It functions as a component of lipoprotein particles which play essential roles in fatty acid and cholesterol transport and metabolism. Alternative splicing generates a short isoform that lacks the N-terminal 72 amino acids. APOM is produced primarily by hepatocytes but also by renal tubule epithelial cells. The signal peptide is not cleaved and is required for APOM association with lipoprotein particles as well as Megalin mediated reabsorption by the kidney. The activity of recombinant mouse APOM was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in APOM. APOM was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 400, 200, 100, 30, 10, 3, 1 and 0.5 µM in 95% ethanol. Mixing 112.5 µL of 50 µg/mL rmAPOM and 12.5 µL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 µL of 50 µg/mL rmAPOM and 12.5 µL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >300 µM.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Joint Bone Spine Apolipoprotein m (APOM) levels and APOM rs805297 G/T polymorphism are associated with increased risk ofrheumatoid arthritis Pubmed: 23660425
Apoptosis The miR-573/apoM/Bcl2A1-dependent signal transduction pathway is essential for hepatocyte apoptosis and hepatocarcinogenesis PubMed: 26201458
Lipids in Health and Disease Effects of hyperlipidaemia on plasma apolipoprotein M levels in patients with type 2 diabetes mellitus: an independent case–control study pubmed:27633510
Lipids in Health and Disease A single-nucleotide polymorphism C-724 /del in the proter region of the apolipoprotein M gene is associated with type 2 diabetes mellitus pubmed:27576735
clinica chimica acta ApoM/HDL-C and apoM/apoA-I ratios are indicators of diabetic nephropathy in healthy controls and type 2 diabetes mellitus. pubmed:28073663
International Journal of Clinical and Experimental Pathology Level of serum apolipoprotein m and its diagnostic significance in inflammatory bowel disease files:ijcep0033416.pdf
Scandinavian Journal of Medicine & Science in Sports Endurance training selectively increases HDL-bound sphingosine-1-phosphate in the plasma pubmed:28493600
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY HDL-S1P Protects Endothelial Function and Reduces Lung Injury During Sepsis in vivo and in vitro Pubmed: 32750426
Biology-Basel Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy 33374530
researchsquare Apolipoprotein M in High Density Lipoprotein Protects Against Astrocyte Apoptosis Induced By Ischemic Insult Via Sphingosine 1-Phosphate Signaling
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC299Mu01 Recombinant Apolipoprotein M (APOM) Positive Control; Immunogen; SDS-PAGE; WB.
APC299Mu01 Active Apolipoprotein M (APOM) Cell culture; Activity Assays.
PAC299Mu01 Polyclonal Antibody to Apolipoprotein M (APOM) WB; IHC; ICC; IP.
LAC299Mu71 Biotin-Linked Polyclonal Antibody to Apolipoprotein M (APOM) WB; IHC; ICC.
SEC299Mu ELISA Kit for Apolipoprotein M (APOM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC299Mu Multiplex Assay Kit for Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.