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Active Lysyl tRNA Synthetase (KARS)

KARS2; LysRS; Lysine tRNA Ligase

ACTIVITY TEST

Lysyl-tRNA Synthetase (KARS) is an essential enzyme that catalyzes the attachment of lysine to its cognate tRNA during protein synthesis, ensuring accurate translation of the genetic code. As a member of the aminoacyl-tRNA synthetase (aaRS) family, KARS plays a critical role in maintaining cellular protein homeostasis. Beyond its canonical function, KARS exhibits non-canonical roles in immune signaling, angiogenesis, and viral defense. It exists in both cytoplasmic and mitochondrial forms, contributing to protein synthesis in multiple cellular compartments. KARS binds to AIMP1 as part of the multi-tRNA synthetase complex (MSC), where this interaction helps regulate enzyme activity and coordinate cellular processes related to protein synthesis.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human KARS and recombinant mouse AIMP1. Briefly, KARS was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AIMP1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KARS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human KARS and recombinant mouse AIMP1 was shown in Figure 1, the EC50 for this effect is 0.015ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APD002Hu01 Active Lysyl tRNA Synthetase (KARS) Cell culture; Activity Assays.
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