≡

Active Phospholipase A2 Receptor 1 (PLA2R1)

CLEC13C; PLA2G1R; PLA2IR; PLA2R; 180 kDa secretory phospholipase A2 receptor; C-type lectin domain family 13 member C; Soluble secretory phospholipase A2 receptor

ACTIVITY TEST

Phospholipase A2 Receptor 1 (PLA2R1) is a type I transmembrane glycoprotein belonging to the mannose receptor family. It is primarily expressed on the surface of podocytes in the kidney glomerulus and plays important roles in cell senescence, apoptosis, and maintaining glomerular filtration barrier function. PLA2R1 has gained significant clinical attention as it is identified as the major autoantigen in primary membranous nephropathy, where autoantibodies against PLA2R1 are pathogenic drivers of the disease. Besides, PLA2R1 functions as a specific receptor that binds secreted PLA2G2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PLA2R1 and recombinant human PLA2G2. Briefly, PLA2G2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PLA2R1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PLA2G2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. When Recombinant PLA2R1 is lmmobilized at 2 µg/mL(100 uLwell), the concentration of PLA2G2 that produces 50% optimal bindingresponse is found to be approximately 0.12 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APD851Hu01 Active Phospholipase A2 Receptor 1 (PLA2R1) Cell culture; Activity Assays.
RPD851Hu01 Recombinant Phospholipase A2 Receptor 1 (PLA2R1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD851Hu01 Polyclonal Antibody to Phospholipase A2 Receptor 1 (PLA2R1) WB
LAD851Hu81 FITC-Linked Polyclonal Antibody to Phospholipase A2 Receptor 1 (PLA2R1) WB; IHC; ICC; IF.
MAD851Hu21 Monoclonal Antibody to Phospholipase A2 Receptor 1 (PLA2R1) WB
SED851Hu ELISA Kit for Phospholipase A2 Receptor 1 (PLA2R1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD851Hu Multiplex Assay Kit for Phospholipase A2 Receptor 1 (PLA2R1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.