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Active Cathepsin G (CTSG)

CG; CTS-G

ACTIVITY TEST

Cathepsin G (CTSG, D993Mu) is a 26 kDa serine protease of the peptidase S1 (chymotrypsin) family, primarily stored in the azurophil granules of neutrophilic polymorphonuclear leukocytes. Encoded by the CTSG gene on chromosome 14q11.2, it is synthesized as a preproprotein and proteolytically processed to yield the active mature enzyme. As a key immune effector, CTSG participates in phagocytic pathogen killing and digestion, mediates connective tissue remodeling at inflammatory loci, and exerts direct antimicrobial activity against pathogens like Staphylococcus aureus. Beyond innate immunity, it modulates immune signaling, tissue repair, and pathological processes including tumor invasion and chronic inflammation. Functionally, CTSG directly cleaves and activates the latent proenzyme form of MMP9 (A553Ca), converting pro-MMP9 into its catalytically active matrix metalloproteinase.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse CTSG and recombinant dog MMP9. Briefly, CTSG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MMP9-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CTSG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse CTSG and recombinant dog MMP9 was shown in Figure 1, the EC50 for this effect is 0.017 µg/mL.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. pubmed:28152632
Lung Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis Pubmed: 31115649
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APD993Mu01 Active Cathepsin G (CTSG) Cell culture; Activity Assays.
RPD993Mu02 Recombinant Cathepsin G (CTSG) Positive Control; Immunogen; SDS-PAGE; WB.
RPD993Mu01 Recombinant Cathepsin G (CTSG) Positive Control; Immunogen; SDS-PAGE; WB.
PAD993Mu01 Polyclonal Antibody to Cathepsin G (CTSG) WB; IHC
PAD993Mu02 Polyclonal Antibody to Cathepsin G (CTSG) WB; IHC; ICC; IP.
LAD993Mu71 Biotin-Linked Polyclonal Antibody to Cathepsin G (CTSG) WB; IHC; ICC.
LAD993Mu81 FITC-Linked Polyclonal Antibody to Cathepsin G (CTSG) WB; IHC; ICC; IF.
MED993Mu Mini Samples ELISA Kit for Cathepsin G (CTSG) Enzyme-linked immunosorbent assay for Antigen Detection.
SED993Mu ELISA Kit for Cathepsin G (CTSG) Enzyme-linked immunosorbent assay for Antigen Detection.
HED993Mu High Sensitive ELISA Kit for Cathepsin G (CTSG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD993Mu Multiplex Assay Kit for Cathepsin G (CTSG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.