≡

Active Cyclin Dependent Kinase Inhibitor 1B (CDKN1B)

CDKN4; KIP1; P27KIP1; p27; Cyclin-dependent kinase inhibitor p27

ACTIVITY TEST

Epidermal Growth Factor (EGF) is a core member of the EGF superfamily of peptide growth factors, a conserved family with EGF-like structural domains. As a potent mitogen, EGF exerts pivotal biological functions by binding to its transmembrane receptor EGFR (ErbB1) and triggering downstream signaling cascades like Ras-MAPK and PI3K-Akt. It robustly promotes the proliferation of epithelial, mesenchymal and neural cells, accelerates cell migration and tissue regeneration, and modulates cell differentiation. Notably, EGF does not induce apoptosis; instead, it suppresses apoptotic pathways to enhance cell survival, playing indispensable roles in embryonic development, wound healing and normal tissue homeostasis.To test the effect of EGF on cell proliferation of Balb/3T3, Balb/3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4, 000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant human EGF with serum-free standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate，then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C. The dose-effect curve of EGF was shown in Figure 1. It was obvious that EGF significantly promoted cell proliferation of Balb/3T3 cells .The ED50 is 0.836 ng/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPE625Hu61 Eukaryotic Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) Positive Control; Immunogen; SDS-PAGE; WB.
APE625Hu61 Active Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) Cell culture; Activity Assays.
RPE625Hu01 Recombinant Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) Positive Control; Immunogen; SDS-PAGE; WB.
PAE625Hu01 Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) WB; IHC
SEE625Hu ELISA Kit for Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) Enzyme-linked immunosorbent assay for Antigen Detection.
LME625Hu Multiplex Assay Kit for Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.