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Active Tubulin Beta 3 (TUBb3)

MC1R; TUBB4; Tubulin beta-4 chain

ACTIVITY TEST

Tubulin Beta 3 (TUBb3) is a primarily neuron-expressed β-tubulin isotype critical for microtubule dynamics, axonal guidance, and neurodevelopment. As a key cytoskeletal component, it maintains neuronal structural integrity and intracellular transport, serving as a differentiation marker. Aberrant TUBB3 expression correlates with neurological disorders and chemoresistance. Its interaction with kallikrein-related peptidase 5 (KLK5) may modulate KLK5's proteolytic activity in pathological contexts such as tumor metastasis or neural plasticity.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human TUBb3 and recombinant mouse KLK5. Briefly, TUBb3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to KLK5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human TUBb3 and recombinant mouse KLK5 was shown in Figure 1, the EC50 for this effect is 0.03 μg/mL.

USAGE

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE711Hu01 Recombinant Tubulin Beta 3 (TUBb3) Positive Control; Immunogen; SDS-PAGE; WB.
APE711Hu01 Active Tubulin Beta 3 (TUBb3) Cell culture; Activity Assays.
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LAE711Hu81 FITC-Linked Polyclonal Antibody to Tubulin Beta 3 (TUBb3) WB; IHC; ICC; IF.
LAE711Hu71 Biotin-Linked Polyclonal Antibody to Tubulin Beta 3 (TUBb3) WB
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SEE711Hu ELISA Kit for Tubulin Beta 3 (TUBb3) Enzyme-linked immunosorbent assay for Antigen Detection.
LME711Hu Multiplex Assay Kit for Tubulin Beta 3 (TUBb3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.