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Active Complement Component 1, Q Subcomponent C (C1qC)

C1QG; Complement Component 1 q subcomponent,Gamma Polypeptide

ACTIVITY TEST

Complement component 1Q subcomponent C (C1qC) is an important part of the C1 complex in the classical activation pathway of the complement system. C1qC contains multiple domains that recognize and bind to pathogen surfaces or antibody-antigen complexes.C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human C1qC and recombinant mouse C1r. Briefly, C1qC was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C1r-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human C1qC and recombinant mouse C1r was shown in Figure 1, the EC50 for this effect is 0.049ug/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLOS ONE Increased complement C1q level marks active disease in human tuberculosis Pubmed:24647646
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