≡

Active 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1)

EDH17B2; EDHB17; HSD17; SDR28C1; Estradiol 17-Beta-Dehydrogenase 1; Short Chain Dehydrogenase/Reductase Family 28CE,1; 20 alpha-hydroxysteroid dehydrogenase

ACTIVITY TEST

Hydroxysteroid (17β) dehydrogenase 1 (HSD17b1) is a steroidogenic enzyme that especially catalyzes the conversion of low potent 17keto-steroids to highly potent 17β-hydroxysteroids. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. Cytochrome P450 1B1 is involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human HSD17b1 and recombinant human CYP1B1. Briefly, HSD17b1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CYP1B1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HSD17B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human HSD17b1 and recombinant human CYP1B1 was shown in Figure 1, the EC50 for this effect is 0.098 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF171Hu01 Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) Positive Control; Immunogen; SDS-PAGE; WB.
APF171Hu01 Active 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) Cell culture; Activity Assays.
PAF171Hu01 Polyclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) WB; IHC; ICC; IP.
MAF171Hu21 Monoclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) WB; IHC; ICC; IP.
SEF171Hu ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF171Hu Multiplex Assay Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.