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Active Leukocyte Cell Derived Chemotaxin 2 (LECT2)

Chm-II; Chm2

ACTIVITY TEST

Leukocyte Cell-Derived Chemotaxin 2 (LECT2) is a multifunctional protein primarily secreted by hepatocytes, with additional expression in immune cells and endothelial cells. First identified as a chemotactic factor that recruits neutrophils, it is now recognized to exert broader roles across diverse physiological and pathological processes—including liver regeneration, angiogenesis, osteoblastogenesis, and metabolic regulation. Functioning as a cytokine-like signaling molecule, LECT2 can bind to specific receptors (e.g., CD209a) to modulate immune responses. Besides,Alpha-1-Antitrypsin (a1AT) has been identified as an interactor of LECT2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat LECT2 and recombinant human a1AT. Briefly, a1AT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LECT2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-a1AT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. When Recombinant LECT2 is lmmobilized at 2 µg/mL(100 uLwell), the concentration of a1AT that produces 50% optimal bindingresponse is found to be approximately 0.073 µg/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Translational Gastroenterology and Hepatology Leukocyte cell derived chemotaxin-2 (Lect2) as a predictor of survival in adult acute liver failure Pubmed: 30976720
J Endocrinol Leukocyte cell-derived chemotaxin 2 affects nonalcoholic fatty liver disease Pubmed: 32485675
肝脏 血清LECT2 检测对肝细胞癌诊断的意义
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APF541Ra01 Active Leukocyte Cell Derived Chemotaxin 2 (LECT2) Cell culture; Activity Assays.
RPF541Ra01 Recombinant Leukocyte Cell Derived Chemotaxin 2 (LECT2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF541Ra01 Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 2 (LECT2) IHC