≡

Active Peptidyl Arginine Deiminase Type II (PADI2)

PAD-H19; PAD2; PDI2; Peptidylarginine deiminase II

ACTIVITY TEST

Peptidyl Arginine Deiminase Type II (PADI2) is a calcium-dependent enzyme that catalyzes the post-translational deimination of arginine residues to citrulline, playing key roles in epigenetic regulation, immune response, and diseases like rheumatoid arthritis and multiple sclerosis. PADI2 interacts with Carbonic Anhydrase I (CA1), potentially modulating cellular pH balance and metabolic processes through their functional interplay. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human PADI2 and recombinant human CA1. Briefly, PADI2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CA1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PADI2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human PADI2 and recombinant human CA1 was shown in Figure 1, the EC50 for this effect is 0.054ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APF707Hu01 Active Peptidyl Arginine Deiminase Type II (PADI2) Cell culture; Activity Assays.
RPF707Hu01 Recombinant Peptidyl Arginine Deiminase Type II (PADI2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF707Hu01 Polyclonal Antibody to Peptidyl Arginine Deiminase Type II (PADI2) WB; IHC; ICC; IP.
MAF707Hu21 Monoclonal Antibody to Peptidyl Arginine Deiminase Type II (PADI2) WB; IHC; ICC; IP.
SEF707Hu ELISA Kit for Peptidyl Arginine Deiminase Type II (PADI2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF707Hu Multiplex Assay Kit for Peptidyl Arginine Deiminase Type II (PADI2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.