≡

Active Dipeptidyl Peptidase 9 (DPP9)

DPRP2; DPLP9; Dipeptidylpeptidase 9; Dipeptidyl peptidase IV-related protein 2; Dipeptidyl peptidase-like protein 9; Dipeptidyl peptidase IX

ACTIVITY TEST

Dipeptidyl Peptidase 9 (DPP9) is a cytosolic serine protease that cleaves N-terminal dipeptides from substrates, regulating immune responses, cell survival, and energy metabolism. As a member of the DPP4 family, it modulates inflammatory signaling and plays roles in cancer progression and metabolic disorders. DPP9's inhibition of NLRP1 inflammasome activation highlights its importance in immune regulation. DPP9 has been reported to interact with HRAS, potentially influencing MAPK/ERK signaling, though its precise role in RAS-driven tumorigenesis requires further investigationThus a functional ELISA assay was conducted to detect the interaction of recombinant mouse DPP9 and recombinant human HRAS. Briefly, DPP9 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HRAS-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DPP9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse DPP9 and recombinant human HRAS was shown in Figure 1, the EC50 for this effect is 0.06 μg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPG444Mu02 Recombinant Dipeptidyl Peptidase 9 (DPP9) Positive Control; Immunogen; SDS-PAGE; WB.
APG444Mu01 Active Dipeptidyl Peptidase 9 (DPP9) Cell culture; Activity Assays.
RPG444Mu01 Recombinant Dipeptidyl Peptidase 9 (DPP9) Positive Control; Immunogen; SDS-PAGE; WB.
PAG444Mu02 Polyclonal Antibody to Dipeptidyl Peptidase 9 (DPP9) WB; IHC; ICC; IP.
PAG444Mu01 Polyclonal Antibody to Dipeptidyl Peptidase 9 (DPP9) WB; IHC; ICC; IP.
SEG444Mu ELISA Kit for Dipeptidyl Peptidase 9 (DPP9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG444Mu Multiplex Assay Kit for Dipeptidyl Peptidase 9 (DPP9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.