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Active TANK Binding Kinase 1 (TBK1)

NAK; T2K; NF-kappa-B-activating kinase; Serine/threonine-protein kinase TBK1

ACTIVITY TEST

TANK Binding Kinase 1 (TBK1) is a serine/threonine kinase involved in innate immune signaling, particularly in regulating interferon (IFN) production and autophagy. It phosphorylates and activates transcription factors like IRF3/IRF7 to drive antiviral responses. TBK1 also interacts with signaling adaptors (e.g., TANK, TRIF) and is linked to inflammation and neurodegenerative diseases. Dysregulation of TBK1 has been associated with cancer and autoimmune disorders. Besides,FGFR1 has been identified as an interactor of TBK1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TBK1 and recombinant human FGFR1. Briefly, TBK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TBK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human TBK1 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 1.11 μg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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