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Active Pro-Melanin Concentrating Hormone (PMCH)

MCH; Neuropeptide-glycine-glutamic acid

ACTIVITY TEST

Pro-MCH (PMCH), short for Pro-melanin-concentrating hormone, is a precursor protein and it can be cleaved and processed to produce melanin-concentrating hormone, a neuropeptide involved in the regulation of various physiological processes like appetite, sleep-wake cycles and energy balance in organisms. In addition, Pro-MCH and its derivative MCH are also involved in the regulation of mood and stress responses, and have certain effects on the body's adaptation to environmental changes.Neuropeptide Y (NPY) has been identified as an interactor of PMCH, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat NPY and recombinant human PMCH . Briefly, NPY was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PMCH-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NPY pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately.Measured by its binding ability in a functional ELISA. When Recombinant PMCH is lmmobilized at 2 ug/mL(100 uLwell), the concentration of NPY that produces 50% optimal bindingresponse is found to be approximately 0.055ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APH350Hu01 Active Pro-Melanin Concentrating Hormone (PMCH) Cell culture; Activity Assays.
RPH350Hu01 Recombinant Pro-Melanin Concentrating Hormone (PMCH) Positive Control; Immunogen; SDS-PAGE; WB.