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Active Myocilin (MYOC)

GLC1A; GPOA; JOAG; JOAG1; TIGR; Trabecular Meshwork Inducible Glucocorticoid Response; Myocilin 55 kDa subunit

ACTIVITY TEST

Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH). Mutations of the MYOC gene are associated with primary open angle glaucoma (POAG), which is a complex disorder with a major heritable component. Myocilin stimulates cell migration that involves the activation of intergrin focal adhesion kinase (FAK)-serin/threonine kinase (AKT) signaling pathway. To test the effect of MYOC on cell proliferation, AGS cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant mouse MYOC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse MYOC for 72h. The result was shown in Figure 1. It was obvious that MYOC significantly increased cell viability of AGS cells. The ED50 of recombinant mouse MYOC is 1.033μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
EPH586Mu61 Eukaryotic Myocilin (MYOC) Positive Control; Immunogen; SDS-PAGE; WB.
EPH586Mu62 Eukaryotic Myocilin (MYOC) Positive Control; Immunogen; SDS-PAGE; WB.
RPH586Mu01 Recombinant Myocilin (MYOC) Positive Control; Immunogen; SDS-PAGE; WB.
APH586Mu61 Active Myocilin (MYOC) Cell culture; Activity Assays.