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Active Eosinophil Peroxidase (EPX)

EPO; EPP; EPX-PEN; EPER

ACTIVITY TEST

Eosinophil Peroxidase (EPX) is a key enzyme exclusively expressed in eosinophils, a type of white blood cell. It plays a central role in the immune system’s defense against parasitic infections and participates in regulating inflammatory responses. EPX catalyzes the production of hypohalous acids, which exhibit strong antimicrobial activity to eliminate pathogens. Abnormal EPX expression or function is linked to allergic diseases like asthma and chronic eosinophilic disorders. EPX and Ribonuclease A3 (RNASE3) interact in eosinophils; this binding modulates their respective antimicrobial and pro-inflammatory activities to coordinate immune responses.To detect the activity of recombinant EPX , a functional ELISA assay was performed to evaluate the interaction between recombinant human EPX and recombinant human RNASE3.Briefly, EPX was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RNASE3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-EPX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human EPX and human RNASE3
was shown in Figure 1, the EC50 for this effect is 0.042µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
International archives of allergy and immunology Allergen-Induced Increases in Interleukin-25 and Interleukin-25 Receptor Expression in Mature Eosinophils from Atopic Asthmatics. pubmed:27685606
Experimental and Therapeutic Medicine Eosinophilic biomarkers for detection of acute exacerbation of chronic obstructive pulmonary disease with or without pulmonary embolism 10.3892/etm.2017.4876
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPJ138Hu01 Recombinant Eosinophil Peroxidase (EPX) Positive Control; Immunogen; SDS-PAGE; WB.
APJ138Hu01 Active Eosinophil Peroxidase (EPX) Cell culture; Activity Assays.
PAJ138Hu01 Polyclonal Antibody to Eosinophil Peroxidase (EPX) WB; IHC; ICC; IP.
MAJ138Hu21 Monoclonal Antibody to Eosinophil Peroxidase (EPX) WB; IHC; ICC; IP.
SEJ138Hu ELISA Kit for Eosinophil Peroxidase (EPX) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ138Hu Multiplex Assay Kit for Eosinophil Peroxidase (EPX) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.