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Active Steroid 5 Alpha Reductase 2 (SRD5a2)

3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 2

ACTIVITY TEST

Steroid 5 Alpha Reductase 2 (SRD5a2) is a microsomal enzyme critical in steroid metabolism, primarily converting testosterone to dihydrotestosterone (DHT), a potent androgen. Expressed in androgen-sensitive tissues (e.g., prostate, skin), it regulates male sexual development, hair growth, and prostate function. Additionally, the SRD5a2-CYP17A1 complex optimizes androgen synthesis: CYP17A1 generates testosterone precursors, while SRD5a2 amplifies androgen potency via DHT production. This collaboration ensures efficient testosterone utilization and targeted DHT formation, critical for physiological androgen effects and pathological conditions.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human SRD5a2 and recombinant human CYP17A1. Briefly, CYP17A1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SRD5a2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CYP17A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. When recombinant SRD5a2 is lmmobilized at 2 ug/mL(100 uLwell), the concentration of CYP17A1 that produces 50% optimal bindingresponse is found to be approximately 0.307 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPM285Hu03 Recombinant Steroid 5 Alpha Reductase 2 (SRD5a2) Positive Control; Immunogen; SDS-PAGE; WB.
RPM285Hu04 Recombinant Steroid 5 Alpha Reductase 2 (SRD5a2) Positive Control; Immunogen; SDS-PAGE; WB.
APM285Hu03 Active Steroid 5 Alpha Reductase 2 (SRD5a2) Cell culture; Activity Assays.
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SEM285Hu ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMM285Hu Multiplex Assay Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.