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Eukaryotic Collagen Type II (COL2)

  • Product No.EPA572Rb61
  • Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
  • SourceEukaryotic expression
  • Host293F Cell
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Predicted Molecular Mass27.81kDa
  • Accurate Molecular Mass33kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla1253~Leu1487 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • Downloadn/a
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$   US$   US$   US$   US$  
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
Spine (Phila Pa 1976). No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes. Pubmed:25856264
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Materials Science and Engineering: C Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition Pubmed:24433895
義守大學生物技術與化學工程研究所學位論文 可拋棄式退化性關節炎電化學式尿液免疫感測器之研究 Electrochemical Immunosensor Detection of Urinary Biomaker for Osteoarthritis Diagnosis Publication: U0074-2807201500521300
Biomed Rep Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs PubMed: 26623021
Macromolecular Bioscience Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063
中国眼耳鼻喉科杂志 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响 article:11593
Biomedical Materials An innovative co-axial system to electrospin in situ crosslinked gelatin nanofibers. Pubmed:26987305
Scientific Reports Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds pubmed:27821864
PLoS One. Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788
Polymers Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering 2073-4360:8
PLOS ONE Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders 10.1371:journal.pone.0187564
PLoS One Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders 10.1371:journal.pone.0172181
Platelets. Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate. pubmed:29090639
Molecular Medicine Reports Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits Pubmed:29257344
Journal of Materials Science Scaffolds modified with graphene as future implants for nasal cartilage
Processes A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
EPA572Rb61 Eukaryotic Collagen Type II (COL2) Positive Control; Immunogen; SDS-PAGE; WB.
EPA572Rb62 Eukaryotic Collagen Type II (COL2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA572Rb51 Polyclonal Antibody to Collagen Type II (COL2) WB
LAA572Rb71 Biotin-Linked Polyclonal Antibody to Collagen Type II (COL2) WB; IHC; ICC.
SEA572Rb ELISA Kit for Collagen Type II (COL2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA572Rb Multiplex Assay Kit for Collagen Type II (COL2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.