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Recombinant Histone H4 (H4)

  • Product No.RPA289Mi01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method).
  • Subcellular LocationSecreted
  • Predicted Molecular Mass41.3kDa
  • Accurate Molecular Mass44kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer2~Gly103 with N-terminal His and GST Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 80%
  • Isoelectric Point12.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 164  US$ 410  US$ 820  US$ 2460  US$ 6150 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One. N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice Pubmed:24816808
Crit Care. Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation Pubmed:25260379
Biomed Res Int A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649891
Journal of Viral Hepatitis Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation pubmed:27660136
Cell Death & Disease Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis Pubmed: 30778043
Respir Res Histone H4 induces heparan sulfate degradation by activating heparanase in chlorine gas-induced acute respiratory distress syndrome Pubmed:35073921
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA289Mi02 Recombinant Histone H4 (H4) Positive Control; Immunogen; SDS-PAGE; WB.
RPA289Mi01 Recombinant Histone H4 (H4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA289Mi02 Polyclonal Antibody to Histone H4 (H4) WB; IHC; ICC; IP.
PAA289Mi01 Polyclonal Antibody to Histone H4 (H4) WB
SEA289Mi ELISA Kit for Histone H4 (H4) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA289Mi High Sensitive ELISA Kit for Histone H4 (H4) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA289Mi CLIA Kit for Histone H4 (H4) Chemiluminescent immunoassay for Antigen Detection.
LMA289Mi Multiplex Assay Kit for Histone H4 (H4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.