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Recombinant Creatine Kinase MB Isoenzyme (CKMB)

CK2; CK-MB; Creatine Kinase,Muscle/Brain

  • Product No.RPA479Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Predicted Molecular Mass76kDa
  • Accurate Molecular Mass73kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Lys381 with N-terminal His and GST Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • Downloadn/a
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$   US$   US$   US$   US$  
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cardiovascular Toxicology Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion Injury PubMed: 22814936
PLoS One N-Acetylcysteine and Allopurinol Confer Synergy in Attenuating Myocardial Ischemia Injury via Restoring HIF-1α/HO-1 Signaling in Diabetic Rats PubMed: PMC3715528
Int J Clin Exp Med Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbits PubMed: 26629064
European Journal of Pharmacology Diabetes blocks the cardioprotective effects of sevoflurane postconditioning by impairing Nrf2/Brg1/HO-1 signaling Pubmed:26973173
Asian Pac J Trop Med.  Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect Pubmed:26972400
Heart International Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction pubmed:27924211
American Journal of Translational Research Peroxisome proliferator-activated receptor γ (PPARγ) mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via … PMC5209473
Archivum Immunologiae et Therapiae Experimentalis In Vivo Cardioprotective Effects and Pharmacokinetic Profile of N-Propyl Caffeamide AgainstIschemia Reperfusion Injury. pubmed:27480649
Archivos de medicina veterinaria Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004
Cellular Physiology and Biochemistry Remote Limb Ischaemic Postconditioning Protects Against Myocardial Ischaemia/ Reperfusion Injury in Mice: Activation of JAK/STAT3-Mediated Nrf2-Antioxidant Signalling 10.1159:481755
Erciyes Medical Journal Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in Rats DOI: 10.5152/etd.2017.1732
International Immunopharmacology Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK pubmed:28410530
Science of the Total Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and Pubmed:29727991
Journal of Surgical Research GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice Pubmed:29605032
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781
Cell Death Discovery Cyclic helix B peptide ameliorates acute myocardial infarction in mice by inhibiting apoptosis and inflammatory responses
Oxidative Medicine and Cellular Longevity Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 Activation Pubmed: 32190173
PLoS One Tissue suction-mediated gene transfer to the beating heart in mice Pubmed: 32027678
FASEB JOURNAL Deletion of Rap1 protects against myocardial ischemia/reperfusion injury through suppressing cell apoptosis via activation of STAT3 signaling Pubmed: 32020680
Molecular Medicine Reports Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury Pubmed: 32705277
In Vivo Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heart Pubmed: 32871779
Drug Design Development and Therapy Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 Cells Pubmed: 32546969
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles Pubmed: 32340313
Molecular Medicine Reports The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin?induced cardiotoxicity 33300057
Oxid Med Cell Longev Inactivation of TOPK Caused by Hyperglycemia Blocks Diabetic Heart Sensitivity to Sevoflurane Postconditioning by Impairing the PTEN/PI3K/Akt Signaling 33986917
Eur J Pharmacol Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R … Pubmed:34968461
Cardiovasc Drugs Ther Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic … Pubmed:35715527
Nutrients Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 Pubmed:35215383
Experimental and Therapeutic Medicine Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest Pubmed:35495584
Oxidative Medicine and Cellular Longevity MicroRNA-503 Exacerbates Myocardial Ischemia/Reperfusion Injury via Inhibiting PI3K/Akt-and STAT3-Dependent Prosurvival Signaling Pathways Pubmed:35620576
International Journal of Sciences: Basic and Applied Research Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA479Mu01 Recombinant Creatine Kinase MB Isoenzyme (CKMB) Positive Control; Immunogen; SDS-PAGE; WB.
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LMA479Mu Multiplex Assay Kit for Creatine Kinase MB Isoenzyme (CKMB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.