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Active Coagulation Factor VII (F7)

FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa

ACTIVITY TEST

The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Am J Physiol Heart Circ Physiol. Monocytes regulate systemic coagulation and inflammation in abdominal sepsis Pubmed:25502108
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277
Cardiovascular Research Loss of accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity Pubmed:29444200
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB874Ra03 Recombinant Coagulation Factor VII (F7) Positive Control; Immunogen; SDS-PAGE; WB.
RPB874Ra01 Recombinant Coagulation Factor VII (F7) Positive Control; Immunogen; SDS-PAGE; WB.
RPB874Ra02 Recombinant Coagulation Factor VII (F7) Positive Control; Immunogen; SDS-PAGE; WB.
APB874Ra02 Active Coagulation Factor VII (F7) Cell culture; Activity Assays.
PAB874Ra01 Polyclonal Antibody to Coagulation Factor VII (F7) WB; IHC; ICC; IP.
PAB874Ra02 Polyclonal Antibody to Coagulation Factor VII (F7) WB; IHC; ICC; IP.
LAB874Ra71 Biotin-Linked Polyclonal Antibody to Coagulation Factor VII (F7) WB; IHC; ICC.
SEB874Ra ELISA Kit for Coagulation Factor VII (F7) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB874Ra Multiplex Assay Kit for Coagulation Factor VII (F7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.