ELISA Kit for Mucin 1 (MUC1)

CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

Specificity

This assay has high sensitivity and excellent specificity for detection of Mucin 1 (MUC1).
No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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MagazineReference
Annals of surgical oncologyPancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
J Hum LactHost Defense Proteins in Breast Milk and Neonatal Yeast ColonizationPubMed: 26116637
BreastActivated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive IndicatorPubMed: 26446295
PLoS One.The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital FluidsPubmed:27182834
Int J Clin Exp MedThe influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghaifiles:ijcem0020163.pdf
TalantaUltrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification.pubmed:28107977
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