ELISA Kit for Mucin 1 (MUC1)
CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin
- Product No.SEA413Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.266ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 560
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For more details, please contact local distributors! US$ 39200
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Mucin 1 (MUC1).
No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 1 (MUC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Annals of surgical oncology||Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734|
|J Hum Lact||Host Defense Proteins in Breast Milk and Neonatal Yeast Colonization PubMed: 26116637|
|Breast||Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator PubMed: 26446295|
|PLoS One.||The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital Fluids Pubmed:27182834|
|Int J Clin Exp Med||The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai files:ijcem0020163.pdf|
|Talanta||Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification. pubmed:28107977|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA413Hu01||Recombinant Mucin 1 (MUC1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA413Hu02||Recombinant Mucin 1 (MUC1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA413Hu03||Recombinant Mucin 1 (MUC1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPA413Hu21||OVA Conjugated Mucin 1 (MUC1)||Immunogen; SDS-PAGE; WB.|
|SPA413Hu02||Synthetic Mucin 1 (MUC1)||Immunogen; Blocking Peptide.|
|PAA413Hu01||Polyclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC; IP.|
|PAA413Hu08||Polyclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC; IP.|
|PAA413Hu02||Polyclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC; IP.|
|PAA413Hu03||Polyclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC; IP.|
|LAA413Hu81||FITC-Linked Polyclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC; IF.|
|LAA413Hu71||Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC.|
|MAA413Hu22||Monoclonal Antibody to Mucin 1 (MUC1)||WB; IHC; ICC; IP.|
|SEA413Hu||ELISA Kit for Mucin 1 (MUC1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA413Hu01||ELISA Kit DIY Materials for Mucin 1 (MUC1)||Main materials for "Do It (ELISA Kit) Yourself".|