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Active Heparin Cofactor II (HCII)

SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2

ACTIVITY TEST

Heparin Cofactor II (HCII) is a plasma serine protease inhibitor synthesized mainly in the liver. Circulating freely in blood plasma, it serves as a key regulator of the coagulation cascade. Unlike antithrombin, HCII selectively suppresses thrombin rather than multiple clotting proteases. Its inhibitory activity remains weak under physiological conditions; binding to glycosaminoglycans such as heparin sodium induces conformational rearrangement of HCII, exposing its reactive site loop to rapidly trap thrombin. HCII limits excessive fibrin generation and prevents pathological thrombosis, maintaining the balance between hemostasis and anticoagulation in the vasculature. HCII binds Hep-Na via positively charged residues, triggering structural shift to accelerate thrombin inhibition. Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat HCII and Hep-Na. Briefly, HCII was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μL were then transferred to Hep-Na-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HCII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat HCII and Hep-Na was shown in Figure 1, the EC50 for this effect is 2.56µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Immunology Proteolytic Activation Transforms Heparin Cofactor II into a Host Defense Molecule PubMed: PMC3677170
Biomacromolecules Anticoagulant properties of poly (sodium 2-(acrylamido)-2-methylpropanesulfonate)-based di-and triblock polymers Pubmed:29733637
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA284Ra01 Active Heparin Cofactor II (HCII) Cell culture; Activity Assays.
RPA284Ra01 Recombinant Heparin Cofactor II (HCII) Positive Control; Immunogen; SDS-PAGE; WB.
PAA284Ra01 Polyclonal Antibody to Heparin Cofactor II (HCII) WB
MAA284Ra21 Monoclonal Antibody to Heparin Cofactor II (HCII) WB; IHC; ICC; IP.
SEA284Ra ELISA Kit for Heparin Cofactor II (HCII) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA284Ra Multiplex Assay Kit for Heparin Cofactor II (HCII) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.