Packages (Simulation)

Polyclonal Antibody to Heparin Cofactor II (HCII)

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Western Blot; Sample: Rat Plasma Primary Ab: 0.3µg/ml Rabbit Anti-Rat HCII Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 2µg/mL Rabbit Anti-Rat HCII Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Lung lysate;
Primary Ab: 2µg/mL Rabbit Anti-Rat HCII Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Heparin-Cofactor-II--HCII--PAA284Ra01.pdf

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Polyclonal Antibody to Heparin Cofactor II (HCII)

SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2

Product No.PAA284Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA284Ra01-Recombinant Heparin Cofactor II (HCII)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration1mg/ml
Organism Species Moren/a
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 103 239 US$ 239 US$ 342 US$ 855 US$ 3420
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HCII. It has been selected for its ability to recognize HCII in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Journal of Immunology	Proteolytic Activation Transforms Heparin Cofactor II into a Host Defense Molecule PubMed: PMC3677170
Biomacromolecules	Anticoagulant properties of poly (sodium 2-(acrylamido)-2-methylpropanesulfonate)-based di-and triblock polymers Pubmed:29733637

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
APA284Ra01	Active Heparin Cofactor II (HCII)	Cell culture; Activity Assays.
RPA284Ra01	Recombinant Heparin Cofactor II (HCII)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA284Ra01	Polyclonal Antibody to Heparin Cofactor II (HCII)	WB
MAA284Ra21	Monoclonal Antibody to Heparin Cofactor II (HCII)	WB; IHC; ICC; IP.
SEA284Ra	ELISA Kit for Heparin Cofactor II (HCII)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA284Ra	Multiplex Assay Kit for Heparin Cofactor II (HCII) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
CMA284Ra	Multiplex Assay Kit for Heparin Cofactor II (HCII) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.