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Active Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- Product No.APA929Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.4
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
Retinol Binding Protein 4 (RBP4) is a 21-kDa secreted glycoprotein belonging to the lipocalin family, predominantly synthesized in the liver and adipose tissue. It functions as the primary carrier of retinol (vitamin A) in the bloodstream, facilitating retinol transport to target tissues and regulating systemic vitamin A homeostasis. RBP4 plays critical roles in vision, epithelial cell differentiation, immune function, and metabolic regulation, with dysregulation linked to obesity, type 2 diabetes, and cardiovascular diseases. RBP4 binds to retinoic acid-BSA conjugate, enabling targeted delivery of retinoic acid and enhancing its bioavailability in biological systems.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human RBP4 and recombinant human retinoic acid-BSA conjugate. Briefly, RBP4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to retinoic acid-BSA conjugate-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RBP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat RBP4 and recombinant human retinoic acid-BSA conjugate was shown in Figure 1, the EC50 for this effect is 13.88µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.10 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078 |
| Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593 |
| Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats PubMed: PMC3368504 |
| Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles Pubmed:24501381 |
| J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061 |
| Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats Pubmed:25445608 |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126 |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome PubMed: 26351173 |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone Pubmed:27446311 |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. pubmed:27446311 |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858 |
| BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis Pubmed:29386009 |
| Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans Pubmed:29335416 |
| Molecular Medicine Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway Pubmed:30015949 |
| Relationship between retinol and risk of diabetic retinopathy: a case-control study | |
| Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle Pubmed: 31147589 |
| Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals | |
| Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma | |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861 |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274 |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309 |
| Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers 34773507 |