Packages (Simulation)

Polyclonal Antibody to Nischarin (NISCH)

Image (I)

DAB staining on IHC-P; Samples: Human Brain Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NISCH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NISCH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: U2OS cell
Primary Ab: 20µg/ml Rabbit Anti-Human NISCH Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Western Blot; Sample: Jurkat cell lysate Primary Ab: 0.2µg/ml Rabbit Anti-Human NISCH Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Nischarin--NISCH--PAA249Hu01.pdf

Certificate

Polyclonal Antibody to Nischarin (NISCH)

IRAS; Imidazoline Receptor Candidate; I-1 Receptor Candidate Protein; Imidazoline Receptor Antisera Selected; Imidazoline receptor antisera-selected protein

Product No.PAA249Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA249Hu01-Recombinant Nischarin (NISCH)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NISCH. It has been selected for its ability to recognize NISCH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Molecular Medicine Reports	Expression of integrin-binding protein Nischarin in metastatic breast cancer Pubmed:25695373

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA249Hu01	Recombinant Nischarin (NISCH)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA249Hu01	Polyclonal Antibody to Nischarin (NISCH)	WB; IHC; ICC/IF
MAA249Hu22	Monoclonal Antibody to Nischarin (NISCH)	WB; IHC; ICC; IP.
SEA249Hu	ELISA Kit for Nischarin (NISCH)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA249Hu	Multiplex Assay Kit for Nischarin (NISCH) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
CMA249Hu	Multiplex Assay Kit for Nischarin (NISCH) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.