Packages (Simulation)

Polyclonal Antibody to Heparin Cofactor II (HCII)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human HCII Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Image (II)

Western Blot; Sample: Mouse Plasma Primary Ab: 0.1µg/ml Rabbit Anti-Human HCII Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human HCII Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Serum;
Primary Ab: 1µg/ml Rabbit Anti-Human HCII Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human HCII Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human HCII Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human peripheral blood mononuclear cell
Primary Ab: 0.3µg/ml Rabbit Anti-Human HCII Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Heparin-Cofactor-II-(HCII)-PAA284Hu01.pdf

Certificate

Polyclonal Antibody to Heparin Cofactor II (HCII)

SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2

Product No.PAA284Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA284Hu01-Recombinant Heparin Cofactor II (HCII)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HCII. It has been selected for its ability to recognize HCII in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Journal of Immunology	Proteolytic Activation Transforms Heparin Cofactor II into a Host Defense Molecule PubMed: PMC3677170
Biomacromolecules	Anticoagulant properties of poly (sodium 2-(acrylamido)-2-methylpropanesulfonate)-based di-and triblock polymers Pubmed:29733637

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA284Hu01	Recombinant Heparin Cofactor II (HCII)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA284Hu01	Polyclonal Antibody to Heparin Cofactor II (HCII)	WB; IHC
MAA284Hu22	Monoclonal Antibody to Heparin Cofactor II (HCII)	WB; IHC; ICC; IP.
SEA284Hu	ELISA Kit for Heparin Cofactor II (HCII)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEA284Hu	ELISA Kit for Anti-Heparin Cofactor II Antibody (Anti-HCII)	Enzyme-linked immunosorbent assay for Antibody Detection.
LMA284Hu	Multiplex Assay Kit for Heparin Cofactor II (HCII) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
CMA284Hu	Multiplex Assay Kit for Heparin Cofactor II (HCII) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.