Packages (Simulation)

Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

Image (I)

DAB staining on IHC-P; Samples: Human Prostate cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Pancreatic cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: A549 cell
Primary Ab: 20µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

Figure. Western Blot; Sample: Recombinant HDGF, Human.
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: A549 cell lysate; Lane3: Jurkat cell lysate; Lane4: 293T cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HDGF Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human THP1 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human HDGF Polyclonal Antibody (Catalog PAA624Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Hepatoma-Derived-Growth-Factor-(HDGF)-PAA624Hu01.pdf

Certificate

Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

HMG1L2; High-Mobility Group Protein 1-Like 2

Product No.PAA624Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA624Hu01-Recombinant Hepatoma Derived Growth Factor (HDGF)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HDGF. It has been selected for its ability to recognize HDGF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-5μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine	Citations
European Journal of Pharmacology	Hepatoma-derived growth factor promotes the resistance to anti-tumor effects of nordihydroguaiaretic acid in colorectal cancer cells ScienceDirect: S0014299910007302
J Clin Lab Anal	Association of serum hepatoma‐derived growth factor levels with disease activity in rheumatoid arthritis: A pilot study Pubmed:35500218

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA624Hu01	Recombinant Hepatoma Derived Growth Factor (HDGF)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA624Hu01	Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)	WB; IHC; ICC/IF; FCM
MAA624Hu22	Monoclonal Antibody to Hepatoma Derived Growth Factor (HDGF)	WB; IHC; ICC; IP.
SEA624Hu	ELISA Kit for Hepatoma Derived Growth Factor (HDGF)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA624Hu	Multiplex Assay Kit for Hepatoma Derived Growth Factor (HDGF) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
CMA624Hu	Multiplex Assay Kit for Hepatoma Derived Growth Factor (HDGF) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.