Polyclonal Antibody to Glutamate Cysteine Ligase, Modifier Subunit (GCLM)

GLCLR; GECS; GCL; Gamma Glutamylcysteine Synthetase; Glutamate Cysteine Ligase Regulatory; Gamma-ECS regulatory subunit; Glutamate cysteine ligase modifier subunit

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GCLM. It has been selected for its ability to recognize GCLM in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Functional Foods Effects of maternal serine supplementation on high-fat diet-induced oxidative stress and epigenetic changes in promoters of glutathione synthesis-related genes in … 10.1016:j.jff.2018.05.067
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