Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Mouse Heart Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse VCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse VCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse VCL Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant VCL, Mouse.
Western Blot; Sample:Human Lung lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Vinculin-(VCL)-PAB839Mu01.pdf

Certificate

Polyclonal Antibody to Vinculin (VCL)

MVCL; Metavinculin

Product No.PAB839Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB839Mu01-Recombinant Vinculin (VCL)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreHomo sapiens (Human), Rattus norvegicus (Rat)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 110 258 US$ 258 US$ 368 US$ 920 US$ 3680
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
PBMCs S-NO proteomic signature in heart failure	S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure journals:1384523
J Cell Sci	PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals Pubmed:35044463

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPB839Mu01	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB839Mu02	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB839Mu02	Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
PAB839Mu01	Polyclonal Antibody to Vinculin (VCL)	WB; IHC
SEB839Mu	ELISA Kit for Vinculin (VCL)	Enzyme-linked immunosorbent assay for Antigen Detection.
CMB839Mu	Multiplex Assay Kit for Vinculin (VCL) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.
LMB839Mu	Multiplex Assay Kit for Vinculin (VCL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.