Packages (Simulation)
Image (I)
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Figure: FITC staining on IHC-P; Sample: Hela cells.
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DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
Image (II)
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Western Blot; Samples: Lane1: Porcine Cerebrum lysate; Lane2: Hela cell lysate; Lane3: 293T cell lysate; Primary Ab: 0.2µg/ml Rabbit Anti-Human ATXN10 Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ATXN10 Polyclonal Antibody (Catalog PAD248Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Ataxin 10 (ATXN10)
E46L; SCA10; Spinocerebellar Ataxia 10; Brain protein E46 homolog
- Product No.PAD248Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPD248Hu01-Recombinant Ataxin 10 (ATXN10)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ATXN10. It has been selected for its ability to recognize ATXN10 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Molecular Metabolism | Ataxin-10 is part of a cachexokine cocktail triggering cardiac metabolic dysfunction in cancer cachexia Science: Article |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD248Hu01 | Recombinant Ataxin 10 (ATXN10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD248Hu01 | Polyclonal Antibody to Ataxin 10 (ATXN10) | WB; IHC; ICC/IF; FCM |
| MAD248Hu21 | Monoclonal Antibody to Ataxin 10 (ATXN10) | WB; IHC; ICC; IP. |
| SED248Hu | ELISA Kit for Ataxin 10 (ATXN10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CMD248Hu | Multiplex Assay Kit for Ataxin 10 (ATXN10) ,etc. by CBA (Cytometric Bead Array) | FLIA Kit for Antigen Detection. |
| LMD248Hu | Multiplex Assay Kit for Ataxin 10 (ATXN10) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |