High Sensitive ELISA Kit for Cardiac Troponin I (cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
- Product No.HEA478Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.0pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 616
For more details, please contact local distributors! US$ 2772
For more details, please contact local distributors! US$ 5236
For more details, please contact local distributors! US$ 43120
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Cardiac Troponin I (cTnI).
No significant cross-reactivity or interference between High Sensitive Cardiac Troponin I (cTnI) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Cardiac Troponin I (cTnI) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Cardiac Troponin I (cTnI) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Cardiac Troponin I (cTnI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Cardiac Troponin I (cTnI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Cardiac Troponin I (cTnI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Animal and Feed Sciences,||The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source|
|Interact Cardiovasc Thorac Surg.||Comparison of femoral and aortic remote ischaemia preconditioning for cardioprotection against myocardial ischaemia/reperfusion injury in a rat model Pubmed:25205781|
|Exp Ther Med||Cardioprotective effect of remote preconditioning of trauma and remote ischemia preconditioning in a rat model of myocardial ischemia/reperfusion injury PubMed: 26136887|
|Heart International||Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction pubmed:27924211|
|Archivos de medicina veterinaria||Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004|
|PLOS Pathogens||Cathepsin B aggravates coxsackievirus B3-induced myocarditis through activating the inflammasome and promoting pyroptosis Pubmed:29360865|
|Science of the Total Environment||Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and Pubmed:29727991|
|Molecules||Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy Pubmed:29547569|
|Molecules||The Multivariate Regression Statistics Strategy to Investigate Content-Effect Correlation of Multiple Components in Traditional Chinese Medicine Based on a … Pubmed:29494523|
|Molecular Medicine Reports||Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473|
|Molecular Medicine Reports||Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781|
|Biological & Pharmaceutical Bulletin||Protective Effects of Shenfu Injection against Myocardial Ischemia-Reperfusion Injury via Activation of eNOS in Rats Pubmed:29910216|
|Oncology Letters||Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of … Pubmed:29928398|
|BMC Pharmacology and Toxicology||Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis Pubmed:29321061|
|FASEB Journal||Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922|
|Frontiers in Pharmacology||Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway Pubmed: 30890937|
|canadian journal of physiology and pharmacology||Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? Pubmed: 30840489|
|Journal of Cellular Physiology||Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes Pubmed: 30982981|
|BIOSENS BIOELECTRON||Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA … Pubmed: 30954926|
|Life Sciences||Protective effects of two novel nitronyl nitroxide radicals on heart failure induced by hypobaric hypoxia Pubmed: 31102744|
|Mikrochim Acta||DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label Pubmed: 31123904|
|Biosensors and Bioelectronics||DNA nanotetrahedron-assisted electrochemical aptasensor for cardiac troponin I detection based on the co-catalysis of hybrid nanozyme, natural enzyme and artificial …|
|Biofactors||MiR‐183‐5p protects rat hearts against myocardial ischemia/reperfusion injury through targeting VDAC1 Pubmed: 31618500|
|Nature Communications||Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb Pubmed: 32161256|
|BIOMEDICINE & PHARMACOTHERAPY||Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535|
|Pharmacol Res||Therapeutic potential of cPLA2 inhibitor to counteract dilated-cardiomyopathy in cholesterol-treated H9C2 cardiomyocyte and MUNO rat Pubmed: 32942017|
|INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES||Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial Infarction in a Preclinical Porcine Model of Ischemic Heart Disease Pubmed: 32605184|
|Eur J Pharmacol||NLRX1 knockout aggravates lipopolysaccharide (LPS)-induced heart injury and attenuates the anti-LPS cardioprotective effect of CYP2J2/11, 12-EET by enhancing … Pubmed: 32574674|
|American Journal of Translational Research||Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and … Pubmed: 32509190|
|Arabian Journal for Science and Engineering||Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats|
|Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes|
|Hum Exp Toxicol||Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats 33882726|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA478Hu02||Recombinant Cardiac Troponin I (cTnI)||Positive Control; Immunogen; SDS-PAGE; WB.|
|EPA478Hu61||Eukaryotic Cardiac Troponin I (cTnI)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA478Hu01||Recombinant Cardiac Troponin I (cTnI)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA478Hu01||Active Cardiac Troponin I (cTnI)||Cell culture; Activity Assays.|
|PAA478Hu01||Polyclonal Antibody to Cardiac Troponin I (cTnI)||WB; IHC; ICC; IP.|
|LAA478Hu71||Biotin-Linked Polyclonal Antibody to Cardiac Troponin I (cTnI)||WB; IHC; ICC.|
|RAA478Hu21||Recombinant Antibody to Cardiac Troponin I (cTnI)||WB; IHC; ICC.|
|MAA478Hu21||Monoclonal Antibody to Cardiac Troponin I (cTnI)||WB; IHC; ICC; IP.|
|MAA478Hu22||Monoclonal Antibody to Cardiac Troponin I (cTnI)||WB; IHC; ICC; IP.|
|SEA478Hu||ELISA Kit for Cardiac Troponin I (cTnI)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEA478Hu||High Sensitive ELISA Kit for Cardiac Troponin I (cTnI)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|AEA478Hu||ELISA Kit for Anti-Cardiac Troponin I Antibody (Anti-cTnI)||Enzyme-linked immunosorbent assay for Antibody Detection.|
|SCA478Hu||CLIA Kit for Cardiac Troponin I (cTnI)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA478Hu01||ELISA Kit DIY Materials for Cardiac Troponin I (cTnI)||Main materials for "Do It (ELISA Kit) Yourself".|