Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 10µg/ml Mouse Anti-Human TNNI3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Samples: Human Skeletal muscle Tissue;
Primary Ab: 10µg/ml Mouse Anti-Human TNNI3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Image (II)
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Western Blot; Sample: Porcine Heart lysate; Primary Ab: 5µg/ml Mouse Anti-Human TNNI3 Antibody Seco
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Western Blot; Sample: Rat Heart lysate;
Primary Ab: 5µg/ml Mouse Anti-Human TNNI3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 5µg/ml Mouse Anti-Human TNNI3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Skeletal muscle lysate; Lane3: Porcine Heart lysate; Lane4: Porcine Skeletal muscle lysate; Lane5: Mouse Heart lysate; Lane6: Canine Heart lysate; Lane7: Bovine Heart lysate;
Primary Ab: 1µg/ml Mouse Anti-Human TNNI3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Quality Guarantee
Instruction Manual
Certificate
Monoclonal Antibody to Cardiac Troponin I (cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
- Product No.MAA478Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)D2
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA478Hu01-Recombinant Cardiac Troponin I (cTnI)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 90
For more details, please contact local distributors! US$ 210
For more details, please contact local distributors! US$ 300
For more details, please contact local distributors! US$ 750
For more details, please contact local distributors! US$ 3000
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against cTnI. It has been selected for its ability to recognize cTnI in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-5µg/mL;1:200-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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PBS Buffer
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DAB Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source |
| Interact Cardiovasc Thorac Surg. | Comparison of femoral and aortic remote ischaemia preconditioning for cardioprotection against myocardial ischaemia/reperfusion injury in a rat model Pubmed:25205781 |
| Exp Ther Med | Cardioprotective effect of remote preconditioning of trauma and remote ischemia preconditioning in a rat model of myocardial ischemia/reperfusion injury PubMed: 26136887 |
| Heart International | Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction pubmed:27924211 |
| Archivos de medicina veterinaria | Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA478Hu02 | Recombinant Cardiac Troponin I (cTnI) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA478Hu01 | Recombinant Cardiac Troponin I (cTnI) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA478Hu01 | Active Cardiac Troponin I (cTnI) | Cell culture; Activity Assays. |
| PAA478Hu01 | Polyclonal Antibody to Cardiac Troponin I (cTnI) | WB; IHC; ICC; IP. |
| LAA478Hu71 | Biotin-Linked Polyclonal Antibody to Cardiac Troponin I (cTnI) | WB; IHC; ICC. |
| MAA478Hu21 | Monoclonal Antibody to Cardiac Troponin I (cTnI) | WB; IHC; ICC; IP. |
| MAA478Hu22 | Monoclonal Antibody to Cardiac Troponin I (cTnI) | WB; IHC; ICC; IP. |
| SEA478Hu | ELISA Kit for Cardiac Troponin I (cTnI) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA478Hu | CLIA Kit for Cardiac Troponin I (cTnI) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA478Hu01 | ELISA Kit DIY Materials for Cardiac Troponin I (cTnI) | Main materials for "Do It (ELISA Kit) Yourself". |