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Human SK-N-SH cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human COL1a2 Polyclonal Antibody (Catalog FAA215Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Anti-Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody
COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain
- Product No.FAA215Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)n/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFCM
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
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For more details, please contact local distributors!
USAGE
Flow cytometry: 20µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Plos one | Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499 |
| J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731 |
| PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955 |
| American Journal of Translational Research | A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441 |
| Sci Rep | The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768 |
| Experimental and Therapeutic Medicine | Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA215Hu01 | Recombinant Collagen Type I Alpha 2 (COL1a2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA215Hu01 | Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; IHC |
| PAA215Hu02 | Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; IHC; ICC; IP. |
| FAA215Hu01 | Anti-Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody | FCM |
| MAA215Hu22 | Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; IHC; ICC; IP. |
| SEA215Hu | ELISA Kit for Collagen Type I Alpha 2 (COL1a2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA215Hu | Multiplex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |