Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Sample: Porcine Cardiac Muscle Tissue Primary Ab: 20µg/ml Mouse Anti-Human TNNT2 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Image (II)
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Human U2OS cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human TNNT2 Monoclonal Antibody (Catalog MAD232Hu29, red histogram) or Isotype control antibody (Catalog IS067-Mu02, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Instruction Manual
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Monoclonal Antibody to Troponin T Type 2, Cardiac (TNNT2)
CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T
- Product No.MAD232Hu29
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)J21
- Ig Isotype IgG2a Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPD232Hu01-Recombinant Troponin T Type 2, Cardiac (TNNT2)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 113
US$ 263
US$ 375
US$ 938
US$ 3750
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against TNNT2. It has been selected for its ability to recognize TNNT2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats PubMed: 21232020 |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats Wiley: source |
| European Journal of Pharmacology | Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats ScienceDirect: S0014299909009984 |
| Journal of translational medicine | The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury PubMed: PMC3694448 |
| Oral Diseases | Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction Pubmed: 22834943 |
| An International Journal of Pharmaceutical Sciences | In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium Pubmed: 23700796 |
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| Liver Int. | Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis Pubmed:24330504 |
| Cardiovasc Toxicol. | Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs Pubmed:24420420 |
| British Journal of Pharmacology | Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise Pubmed:25039894 |
| Chemico-Biological Interactions | Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways Pubmed:26807765 |
| The Journal of Pharmacology and Experimental Therapeuties | The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice Pubmed:27247000 |
| The Journal of Maternal-Fetal & Neonatal Medicine | Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. pubmed:27937003 |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155 |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830 |
| PLoS One | Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model pubmed:28570599 |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus pubmed:29042932 |
| PLoS One | Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury Pubmed: 30192780 |
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| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy Pubmed: 32148580 |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535 |
| Toxics | Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats Pubmed: 32947820 |
| Elife | Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice 33526170 |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species 34536344 |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications 34673993 |
| Experimental and Therapeutic Medicine | Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest Pubmed:35495584 |