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Monoclonal Antibody to Cytochrome P450 1A2 (CYP1A2)

CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

  • Product No.MAD294Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 120  US$ 280  US$ 400  US$ 1000  US$ 4000 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CYP1A2. It has been selected for its ability to recognize CYP1A2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Environmental Monitoring and Assessment Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes Pubmed:25471622
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
 Journal of FisheriesSciences. com Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress b0407ebde042c4f61b728bc11a586e6c
Toxicology Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes Pubmed:29879457
Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD294Ra01 Recombinant Cytochrome P450 1A2 (CYP1A2) Positive Control; Immunogen; SDS-PAGE; WB.
PAD294Ra01 Polyclonal Antibody to Cytochrome P450 1A2 (CYP1A2) WB; ICC/IF
MAD294Ra21 Monoclonal Antibody to Cytochrome P450 1A2 (CYP1A2) WB; IHC; ICC; IP.
SED294Ra ELISA Kit for Cytochrome P450 1A2 (CYP1A2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD294Ra Multiplex Assay Kit for Cytochrome P450 1A2 (CYP1A2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.