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Recombinant Cytochrome P450 1A2 (CYP1A2)

CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

  • Product No.RPD294Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationEndoplasmic reticulum lumen
  • Predicted Molecular Mass33.0KDa
  • Accurate Molecular Mass33kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal192~Glu444 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.1
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 176  US$ 440  US$ 880  US$ 2640  US$ 6600 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Environmental Monitoring and Assessment Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes Pubmed:25471622
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
 Journal of FisheriesSciences. com Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress b0407ebde042c4f61b728bc11a586e6c
Toxicology Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes Pubmed:29879457
Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD294Ra01 Recombinant Cytochrome P450 1A2 (CYP1A2) Positive Control; Immunogen; SDS-PAGE; WB.
PAD294Ra01 Polyclonal Antibody to Cytochrome P450 1A2 (CYP1A2) WB; ICC/IF
MAD294Ra21 Monoclonal Antibody to Cytochrome P450 1A2 (CYP1A2) WB; IHC; ICC; IP.
SED294Ra ELISA Kit for Cytochrome P450 1A2 (CYP1A2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD294Ra Multiplex Assay Kit for Cytochrome P450 1A2 (CYP1A2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.