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Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC)

GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS

  • Product No.RPD759Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMitochondrion, Cytoplasm
  • Predicted Molecular Mass24kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp435~Ser636 (Accession # P19468) with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point5.65
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 188  US$ 470  US$ 940  US$ 2820  US$ 7050 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oxidative Medicine and Cellular Longevity Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects pubmed:28798861
Journal of Functional Foods Effects of maternal serine supplementation on high-fat diet-induced oxidative stress and epigenetic changes in promoters of glutathione synthesis-related genes in … 10.1016:j.jff.2018.05.067
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD759Ra01 Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) Positive Control; Immunogen; SDS-PAGE; WB.
PAD759Ra01 Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC
MAD759Ra21 Monoclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
SED759Ra ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD759Ra Multiplex Assay Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMD759Ra Multiplex Assay Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.