CLIA Kit for Apolipoprotein B (APOB)
Apo-B; FLDB; Apo B-100; Apo B-48
- Product No.SCC003Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range6.86-5,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.84ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB).
No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Apolipoprotein B (APOB) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein B (APOB) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein B (APOB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|Journal of Lipid Research||Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice Jlr: M019463|
|J Clin Endocrinol Metab.?||Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia Pubmed:25781360|
|Biochim Biophys Acta||Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism PubMed: 26515253|
|Am J Cardiovasc Dis||Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice PubMed: 26064792|
|The Journal of Biological Chemistry||Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation Pubmed:27284008|
|United States Patent Application||COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES y2016:0152988.html|
|alcohol and alcoholism||Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. pubmed:27659607|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|NPC003Hu01||Native Apolipoprotein B (APOB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPC003Hu02||Recombinant Apolipoprotein B (APOB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPC003Hu01||Recombinant Apolipoprotein B (APOB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC003Hu02||Polyclonal Antibody to Apolipoprotein B (APOB)||WB; IHC; ICC; IP.|
|PAC003Hu01||Polyclonal Antibody to Apolipoprotein B (APOB)||WB; IHC; ICC; IP.|
|LAC003Hu81||FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB)||WB; IHC; ICC; IF.|
|LAC003Hu71||Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB)||WB; IHC; ICC.|
|MAC003Hu22||Monoclonal Antibody to Apolipoprotein B (APOB)||WB; IHC; ICC; IP.|
|MAC003Hu21||Monoclonal Antibody to Apolipoprotein B (APOB)||WB; IHC; ICC; IP.|
|SEC003Hu||ELISA Kit for Apolipoprotein B (APOB)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEC003Hu||High Sensitive ELISA Kit for Apolipoprotein B (APOB)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC003Hu||CLIA Kit for Apolipoprotein B (APOB)||Chemiluminescent immunoassay for Antigen Detection.|