CLIA Kit for Apolipoprotein B (APOB)

Apo-B; FLDB; Apo B-100; Apo B-48

Specificity

This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB).
No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Apolipoprotein B (APOB) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein B (APOB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-99 96
EDTA plasma(n=5) 83-101 92
heparin plasma(n=5) 93-104 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein B (APOB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 97-104% 79-96% 99-105%
EDTA plasma(n=5) 79-102% 79-101% 80-99% 98-105%
heparin plasma(n=5) 79-101% 95-103% 87-97% 85-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
Journal of Lipid Research Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice Jlr: M019463
J Clin Endocrinol Metab.? Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia Pubmed:25781360
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism PubMed: 26515253
Am J Cardiovasc Dis Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice PubMed: 26064792
The Journal of Biological Chemistry Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation Pubmed:27284008
United States Patent Application COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES y2016:0152988.html
alcohol and alcoholism Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. pubmed:27659607
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPC003Hu01 Native Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu02 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu01 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
PAC003Hu02 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
PAC003Hu01 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Hu81 FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IF.
LAC003Hu71 Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
MAC003Hu22 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
MAC003Hu21 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
SEC003Hu ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
HEC003Hu High Sensitive ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC003Hu CLIA Kit for Apolipoprotein B (APOB) Chemiluminescent immunoassay for Antigen Detection.